The aim of present work was to develop a simple and sensitive, HPTLC method for the quantitative estimation of Tinidazole in its single component tablet formulations 300 mg). Tinidazole was chromatographed on silica Gel 60 F254 TLC plate using Chloroform: Methanol: Ammonia (9:1:0.1 v/v) as mobile phase. Tinidazole in methanol was scanned by Camag TLC scanner 4 with UV visible detector over wavelength range 200 to 400 nm. Tinidazole showed Rf value 0.53 and scanned at 310 nm using camag TLC scanner. The method was validated in terms of linearity (3-9 μg/ml), Precision (intra-day variation 1.26, inter-day variation 1.4), accuracy (83.57 to 91.80%) and specificity. The limit of detection and limit of quantification for tinidazole were found to be 0.15 µg/ml and 0.47 µg/ml, respectively. It can be concluded from the results that the proposed method was accurate, precise and consistent the determination of tinidazole in tablet dosage form. This method was validated as per ICH guideline Q2 (R1). Results suggest that this method can be used for routine estimation of tinidazole in bulk and pharmaceutical dosage forms.
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